MPN-PCR enumeration of Campylobacter spp. in raw chicken meats and by-products
نویسندگان
چکیده
منابع مشابه
Detection of Campylobacter spp. in chicken fecal samples by real-time PCR.
A real-time PCR assay for detecting thermophilic Campylobacter spp. directly in chicken feces has been developed. DNA was isolated from fecal material by using magnetic beads followed by PCR with a prealiquoted PCR mixture, which had been stored at -18 degrees C. Campylobacter could be detected in less than 4 h, with a detection limit of 100 to 150 CFU/ml, in a fecal suspension. A bacterial int...
متن کاملEnumeration and identity of Campylobacter spp. in Italian broilers.
Transmission of Campylobacter to humans has been prominently associated with mishandling or improperly preparing contaminated poultry carcasses. The number of organisms per carcass represents an important measure of human exposure to the agent. Therefore, we wished to estimate this public exposure over 1 yr among Italian broiler carcasses. We sampled 213 broiler carcasses from rinse water sampl...
متن کاملIsolation and identification of Campylobacter spp. and Campylobacter coli from poultry carcasses by conventional culture method and multiplex PCR in Mashhad, Iran
The genus Campylobacter is of great importance to public health because it includes several species that may cause diarrhoea. Poultry and poultry products are known as important sources of human campylobacteriosis. In this study, during the autumn months of 2005, a total of 100 samples from poultry carcasses, representing 20 broiler flocks were obtained by rinse test, after the chilling stage ...
متن کاملCampylobacter contamination in retail poultry meats and by-products in the world: a literature survey.
Campylobacter species are common bacterial pathogens associated with human gastroenteritis worldwide. In North America, Europe and Japan, campylobacteriosis is one of the leading food-borne bacterial illnesses and the consumption of poultry meats and/or by-products is suspected a major cause of the illness. In this survey, we summarized the research papers describing Campylobacter contamination...
متن کاملEnumeration of Salmonella and Campylobacter spp. in environmental farm samples and processing plant carcass rinses from commercial broiler chicken flocks.
A prospective cohort study was performed to evaluate the prevalences and loads of Salmonella and Campylobacter spp. in farm and processing plant samples collected from 55 commercial broiler chicken flocks. Environmental samples were collected from broiler houses within 48 h before slaughter, and carcass rinses were performed on birds from the same flocks at 4 different stages of processing. Sal...
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ژورنال
عنوان ژورنال: Frontiers of Agriculture in China
سال: 2010
ISSN: 1673-7334,1673-744X
DOI: 10.1007/s11703-010-1042-6